![]() Genotypes with good yield potential for the Southeastern U.S. These results provide a basis to further understand the complex inheritance of CBB resistance in Mesoamerican common beans and show that this biotic stress is unrelated to CR resistance, which was evident during a drought period. A total of 89 candidate genes were identified in close vicinity (☑00 kb) to the significant CBB markers, some of which could be directly or indirectly involved in plant defense to diseases. The GWAS detected 14 significant Single-Nucleotide Polymorphism (SNP) markers associated with CBB resistance distributed on five chromosomes, namely Pv02, Pv04, Pv08, Pv10, and Pv11, but no loci for resistance to CR. We also identified adapted common bean genotypes that could be used directly in Southeastern production or that could be good parents in breeding programs for CBB and CR resistance. The analysis of variance revealed the presence of variability in the MDP for the evaluated traits. CBB resistance was also tested with a field isolate in controlled greenhouse conditions. A total of 300 genotypes from the Mesoamerican Diversity Panel (MDP) were evaluated for CBB and CR in field experiments for three years. The goals of this study were (1) to evaluate field-level resistance from the various commercial classes and subgroups represented in the Mesoamerican gene pool as sources for breeding beans for the region and (2) to evaluate genome-wide marker × trait associations (GWAS) using genetic markers for the genotypes. Hence, field-level resistance would be the best management strategy for these diseases. The pathogens for these two diseases can survive long periods in infested soil or on seeds and are difficult to control through pesticides. However, the warm and humid climate of the Southeastern United States is conducive to diseases such as Common Bacterial Blight (CBB) and Charcoal Rot (CR). We discuss the potential link between the pathways induced during bean colonization and genes induced by transcription activator-like effectors (TALEs), as illustrated in other Xanthomonas pathovars.Ĭommon bacterial blight Common bean RNA-Seq Resistance Xanthomonas.The gene pool of Mesoamerican common beans ( Phaseolus vulgaris L.) includes genotypes in the small-to-medium-size seeded dry beans, as well as some snap beans from hotter environments adapted to the Southeastern United States. ![]() This study helps better understanding the mechanisms occurring during the early colonization phase of common bean by Xanthomonas and unveils new actors potentially important for resistance and susceptibility to CBB. ![]() Resistance was linked to upregulation of the salicylic acid pathway and downregulation of photosynthesis and sugar metabolism, while susceptibility was linked to downregulation of resistance genes and upregulation of the ethylene pathway and of genes involved in cell wall modification. phaseoli showed that the susceptible genotype initiated a more intense and diverse biological response than the resistant genotype. Reciprocal BLASTp analysis led to a list of 20,787 homologs between these genotypes and the common bean reference genome (G19833), which provides a solid dataset for further comparative analyses. We sequenced and annotated the genomes of two common bean genotypes being either resistant (BAT93) or susceptible (JaloEEP558) to CBB. To date, transcriptomic studies after CBB infection have been very scarce and the molecular mechanisms underlying susceptibility or resistance are largely unknown. Resistance to CBB is particularly complex as 26 quantitative resistance loci to CBB have been described so far. fuscans is one of the major threats to common bean crops (Phaseolus vulgaris L.). Common bacterial blight (CBB) caused by Xanthomonas phaseoli pv.
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